Our 3D-EXplore Tumoroid Technology
Build a detailed, highly nuanced understanding of the effect your candidate therapeutic exerts on patient tumor tissue, including the tumor microenvironment (TME), with Nilogen’s proprietary 3D-EXplore tumoroid technology.
A TECHNOLOGY UNLIKE ANY OTHER
While there are many different types of 3D cell technologies, Nilogen’s unique 3D-EXplore tumoroid platform provides the most clinically relevant model of patient tumor biology available.
By minimally processing fresh patient tumor tissue into uniformly sized tumoroids and pooling tumoroids before aliquoting into individual wells, we create highly reproducible representations of the complete tumor in multiple assay wells.
The result is a platform that enables replicate studies and/or integratable analysis by multiple methods—think high content imaging and gene expression analysis and multiplex cytokine analysis and multi-parameter flow cytometry —and delivers similar treatment response rates to that seen in patients.
HOW OUR 3D TUMOROID TECHNOLOGY WORKS
Each well delivers highly reproducible representations of the entire heterogeneous tumor complete with intact tumor microenvironment (TME).
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ISOLATION OF FRESH TUMOR TISSUE
We start with freshly isolated tissue ethically sourced from our extensive network of clinics in the US.
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CONVERSION INTO 3D TUMOROIDS
Each fresh tumor is minimally processed using a proprietary mechanical method into uniformly-sized tumoroids of about 150 µm in diameter. No chemical dissociation, propagation or reassembly.
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POOLING AND ALIQUOTING OF TUMOROIDS
To account for the heterogeneity that exists within each tumor, we pool up to 9,000 tumoroids from a single tumor before aliquoting hundreds of tumoroids into individual wells of a multi-well plate, minimising well-to-well variability.
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EX VIVO TREATMENT WITH CANDIDATE THERAPIES
A single tumor can provide enough tumoroids for many study arms with multiple assays per arm, which are then exposed to drug/combinations. Each drug/combination can be assessed using multiple analytical techniques, resulting in a comprehensive, multi-omics view of multiple drugs/combinations in each tumor.
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DRUG RESPONSE PROFILING & BIOMARKER DISCOVERY
Nilogen uses sophisticated bioinformatics and artificial intelligence algorithms to analyze and integrate your data, ultimately delivering a fully analyzed, interpreted, and actionable report of your study.
SEE THE DIFFERENCE IN NILOGEN’S TUMOROIDS
In this example of a pancreatic tumoroid, the extracellular matrix (ECM) is preserved in addition to cell-cell and cell-ECM interactions.
Frequently asked questions
Featured Applications
GET STARTED WITH NILOGEN
Find out how Nilogen’s 3D-EXplore tumoroid technology can advance your oncology program.
Nilogen Posters
See the robustness of our 3D tumoroid technology in our recent conference posters.
