Multiplex Cytokine Analysis

Cytokines including chemokines, interferons, interleukins, lymphokines, and tumor necrosis factors, are all produced by a broad range of cell types. Cytokines have a specific effect on the behavior of neighboring cells by modulating maturation, growth, and the responsiveness of particular cell populations.

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Why is multiplex cytokine analysis important?

Traditional disassociated 2D models and organoids lack an intact tumor microenvironment and disrupt the signals crucial to tumorigenesis and drug response. Nilogen’s 3D-EXplore tumoroids preserve these intricate interactions. At Nilogen we provide a broad range of cytokine assay services from single-plex up to 100-plex panels for multiplex cytokine analysis to assess immune activity and drug mediated responses.
Our cytokine profiling service provides an enhanced approach to study mechanisms of action in living tumor tissue.

Capabilities

Multiplex Cytokine Analysis

We enable the simultaneous analysis of up to 100 cytokines and chemokines. Traditional disassociated 2D models and organoids lack an intact tumor microenvironment and disrupt the signals crucial to tumorigenesis and drug response. Nilogen’s 3D-EXplore tumoroids preserve these intricate interactions. At Nilogen we provide a broad range of cytokine assay services from single-plex up to 100-plex panels for multiplex cytokine analysis to assess immune activity and drug mediated responses. Our cytokine profiling service provides an enhanced approach to study mechanisms of action in living tumor tissue.

Flow Cytometry

Nilogen’s extensive experience in multiparameter flow cytometry matched with our optimized panels enable our clients to evaluate the immune landscape, T-cell activation, checkpoint inhibition, ADCCs, ADC, myeloid function, phagocytosis and cellular proliferation as well as develop and deploy custom panels.

Multiplex Immunohistochemistry

Multiplex Immunohistochemistry/Immunofluorescence (mIHC/IF) provides high-throughput multiplex staining and standardized semi-quantitative analysis for highly reproducible, efficient and cost-effective tissue studies. This technique allows the simultaneous detection of multiple markers on a single tissue section, providing a comprehensive view of tissue composition, pre- and post-treatment.

Scientific Data

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3D tumoroids derived from fresh CRC patient tumors were treated with combinations of a checkpoint inhibitor and cGAS-STING agonists. After 48 hrs in ex vivo culture, supernatants were collected and analyzed by multiplex cytokine assay.

Related Resources

Browse our latest posters and presentations using Nilogen's fresh patient tumoroid technology.

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