High-content 3D image analysis
Nilogen’s high-content 3D image analysis offers a visual and quantitative solution to drug efficacy in fresh patient tumoroids:
- Real-time monitoring of proliferation, cell viability and cell death
- 3D measurement of volume, intensity, distance between neighboring cells and objects
- Receptor occupancy/Antibody binding including bispecifics
- Oncolytic virotherapy monitoring
Multi-color Flow Cytometry Analysis
In-depth evaluation of the tumor microenvironment
Achieve greater insight into the comprehensive biological activity of tumor cells and the immune microenvironment with our intact fresh patient 3D tumoroids and multi-parameter flow cytometry analysis.
Our flow cytometry assays utilize multiple colors to monitor drug mediated changes in the tumor microenvironment to assess treatment response.
We offer optimized pre-designed panels or can custom design multiplex flow panels to help define the mechanism of action and evaluate:
- tumor infiltrating lymphocytes
- differentiation of immune cell subsets
- immune cell activation
- cell proliferation
Multiplex Cytokine Analysis
Communication is key
Cytokines include: chemokines, interferons, interleukins, lymphokines, and tumor necrosis factors, all produced by a broad range of cell types. Cytokines have a specific effect on the behavior of neighboring cells modulating maturation, growth, and the responsiveness of particular cell populations.
Traditional disassociated 2D models lack an intact TME and disrupt the signals crucial to tumorigenesis and drug response. Nilogen’s 3D tumoroid models preserve these intricate interactions. Nilogen offers a broad range of cytokine analyses including single plex up to 50-plex panels to assess immune activity and drug mediated responses.
Nanostring Gene Expression Analysis
The traditional biomarker approach is based on a single analyte measurement. The interactions of the cells that makeup the unique characteristics of the tumor microenvironment (TME) involve many different cells that need to be analyzed.
Due to the dynamic nature of tumors and their infiltrating lymphocytes, it is important to look beyond genetic variation to assess the phenotypic changes in protein and gene expression levels in order to fully characterize the immune response to disease. Nilogen’s complementary assays including multiplex gene expression analysis allow for a side-by-side analysis in the same patient sample. With this approach we can analyze drug mediated changes in the TME and help develop biomarkers for response that can be useful in future clinical applications.
Nanostring’s PanCancer IO 360 Gene Expression Panel is a unique 770 gene expression panel that combines vital components involved in the complex interplay between the tumor, microenvironment and immune response in cancer allowing for a multifaceted characterization of disease biology and interrogation of mechanisms of immune evasion.
Gene expression profiling of the TME after Keytruda treatment
In partnership with Nanostring, Nilogen provides in-house gene expression profiling. Combined with our innovative 3D tumoroid approach you can simultaneously analyze the genetic profile of all the cell subsets found in the heterogenous tumor microenvironment and the impact your drug has on this dynamic environment.
See where your data can lead you next.
Many other panels are also offered, please inquire for more information.
LEARN MORE FROM US
Find out more about all of Nilogen’s platforms and how we put the TME to work for drug development.